human telomerase reverse transcriptase immortalized adipose tissue derived mscs (ATCC)

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ATCC human telomerase reverse transcriptase immortalized adipose tissue derived mscs
Human Telomerase Reverse Transcriptase Immortalized Adipose Tissue Derived Mscs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adipose+tissue/ASC52telo%2C+hTERT+immortalized+adipose+derived+Mesenchymal+stem+cells/10__3390_slash_ijms27104212-132-0-16
Average 96 stars, based on 250 article reviews

human telomerase reverse transcriptase immortalized adipose tissue derived mscs - by Bioz Stars, 2026-09

96/100 stars

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In Vitro:

Article Title: Intrinsic heterogeneity of primary cilia revealed through spatial proteomics.
Article Snippet: .. We analyzed three immortalized primary human cell lines of different tissue origin:: (1) serum-starved hTERT-RPE1 cells from the embryonic retina,29 predominantly employing the internal ciliogenesis pathway5 and representing the best studied in vitro model for human cilia, (2) RPTEC/TERT1 cells30 from the epithelial kidney proximal tubules, to represent a cell type affected in the most common ciliopathy (polycystic kidney disease, PKD), and (3) the mesenchymal, multipotent, stromal ASC52telo cells31 from adipose tissue. hTERT-RPE1 cells were acquired from ATCC and cultivated in DMEM/F12 (Thermo Fisher Scientific Inc, GibcoTM, Cat. no. 11320033) + 10% FBS (Merck KGaA, Sigma-Aldrich, Cat. no. F7524) at 37◦C and 5% CO2. .. ASC52telo and RPTEC/TERT1 cells were acquired from Evercyte GmbH (Vienna, Austria).

Isolation:

Article Title: Senescence-Associated Alterations in Matrisome of Mesenchymal Stem Cells
Article Snippet: .. ASC52telo is an hTERT-immortalized adipose-derived mesenchymal stem cell line exhibiting a fibroblast-like morphology that was isolated in 2006 from the adipose tissue of a woman (ATCC ® SCRC-4000TM). .. The cells were expanded in α-MEM (Gibco, Life Technologies, Carlsbad, CA, USA) with 50 U/mL penicillin-streptomycin (PanEco, Moscow, Russia), and 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA) at standard conditions (5% CO 2 , 37 °C).

Cell Culture:

Article Title: SOX9 is a key component of RUNX2-regulated transcriptional circuitry in osteosarcoma.
Article Snippet: .. Umbilical cord-derived (Cat#: PCS-500-010), adipose tissue-derived (Cat#: PCS-500-011), and bone marrowderived (BM, Cat#: PCS-500-012) MSCs, SAOS2 (Cat#: HTB-85), U2OS (Cat#: HTB-96), HOS (Cat#: CRL-1543), HOS-MNNG (Cat#: CRL-1547), 143B (Cat#: CRL-8303), G292 (Cat#: CRL-1423), and MG63 (Cat#: CRL-1427) cells were purchased from ATCC and cultured per the vendor’s instructions. ..

Article Title: Inter-laboratory multiplex bead-based surface protein profiling of MSC-derived EV preparations identifies MSC-EV surface marker signatures.
Article Snippet: Lab 3 used cord blood-derivedMSCs (CB-MSC;MSC06) andWharton Jelly-derivedMSCs (WJ-MSC;MSC07): CB-MSC (ATCC, PCS-500-010, Umbilical Cord-DerivedMesenchymal Stem Cells, Normal, Human) were cultured in MEM-αmedia (ThermoFisher Scientific) supplementedwith basic fibroblast growth factor (5 ng/mL; SigmaAldrich), 10%FBS (Invitrogen) and 1%Antibiotic-Antimycotic (anti-anti; ThermoFisher Scientific); WJ-MSCs were cultured in DMEM (1 g/L glucose; containing GlutaMAX-I and sodium pyruvate, Invitrogen) supplemented with 1% anti-anti (ThermoFisher Scientific) (Hagey et al., 2023). .. Adipose tissue-MSCs (ATMSC; MSC08) by Lab 4 (ATCC #SCRC-4000, ASC52telo hTERT immortalised adipose derived mesenchymal stem cells) were cultured in MEM-α media (Gibco, without ribonucleotides, without deoxyribonucleotides) supplemented with GlutaMAX-I, 1% penicillin/streptomycin and 10% FBS (Gibco). ..

Derivative Assay:

Article Title: A New Approach for Orbital Wall Reconstruction in a Rabbit Animal Model Using a Hybrid Hydroxyapatite–Collagen-Based Implant
Article Snippet: Electron microscopy (SEM) analysis: The study of particle morphology was carried out with an FEI Quanta 250 electron microscope in high-vacuum (HV) (ESEM) working mode, using a secondary electron detector (ETD), a backscattered electron detector (CBS), and a detector for energy-dispersive spectroscopy (EDS). .. For the cytotoxicity test of the HAp-COL compound (powder), two cell lines were utilized: a fibroblast line (3T3) and a mesenchymal stem cell (MSC) line, derived from adipose tissue (ATCC, PCS-500-011). ..

Article Title: A New Approach for Orbital Wall Reconstruction in a Rabbit Animal Model Using a Hybrid Hydroxyapatite-Collagen-Based Implant.
Article Snippet: Electron microscopy (SEM) analysis: The study of particle morphology was carried out with an FEI Quanta 250 electron microscope in high-vacuum (HV) (ESEM) working mode, using a secondary electron detector (ETD), a backscattered electron detector (CBS), and a detector for energy-dispersive spectroscopy (EDS). .. For the cytotoxicity test of the HAp-COL compound (powder), two cell lines were utilized: a fibroblast line (3T3) and a mesenchymal stem cell (MSC) line, derived from adipose tissue (ATCC, PCS-500-011). ..

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